The journal Catalysis Science & Technology features on its front cover the recently published research article “Simultaneous screening of multiple substrates with an unspecific peroxygenase enabled modified alkane and alkene oxyfunctionalisations”, a collaboration between the research teams of Jun.-Prof. Martin Weissenborn (Leibniz Institute of Plant Biochemistry, Halle (Saale)) and Dr. Marc Garcia-Borràs. In this project, led by PhD students Anja Knorrscheidt (Halle (Saale), Germany) and Jordi Soler (IQCC), a high throughput GC-MS approach was developed, permitting the simultaneous analysis of up to three substrates and six products quantitatively from one reaction mixture. This screening approach was applied to site-saturation libraries of the novel unspecific peroxygenase MthUPO. Using this setup enabled substantial insights from a small mutant library. Enzyme variants were identified exhibiting selective alkene epoxidation and substantially shifted regioselectivities to 2- and 1-octanol formations. Computational modelling rationalised the observed selectivity changes.
The corresponding paper was published recently:
A. Knorrscheidt, J. Soler, N. Hünecke, P. Püllmann, M. Garcia-Borràs, and M.J. Weissenborn
“Simultaneous screening of multiple substrates with an unspecific peroxygenase enabled modified alkane and alkene oxyfunctionalisations”
Catal. Sci. Technol. 2021, 11, 6058-6064
Girona, September 28, 2021
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